5N9J

Core Mediator of transcriptional regulation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97940, 1.90750, 0.97794, 0.97863
Spacegroup name	P 21 21 21
Unit cell lengths	145.864, 211.768, 267.028
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.486 - 3.400
R-factor	0.2343
Rwork	0.233
R-free	0.25600
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	98.486	98.486	3.600
High resolution limit [Å]	3.400	10.150	3.400
Rmerge	0.058	0.027	1.644
Rmeas	0.063	0.030	1.784
Number of reflections	113813	4578	17800
<I/σ(I)>	17.05	54.15	1.06
Completeness [%]	99.5	99.3	97.5
Redundancy	6.643	5.704	6.622
CC(1/2)	0.999	0.998	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293	0.1 M MES pH 6.5, 3.2% PEG 20000, 700 mM ammonium nitrate
1	EVAPORATION	6.5	293	0.1 M MES pH 6.5, 3.2% PEG 20000, 700 mM ammonium nitrate