5N92

Crystal Structure of Human IL-17AF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.999970
Spacegroup name	P 41 2 2
Unit cell lengths	87.380, 87.380, 126.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.2071
Rwork	0.205
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.000	15.000	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.130	0.029	1.493
Rmeas	0.136	0.030	1.554
Total number of observations	278250
Number of reflections	22302	311	1611
<I/σ(I)>	14.67	41.86	1.75
Completeness [%]	99.5	100	100
Redundancy	12.476	10.232	13.171
CC(1/2)	0.999	1.000	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	0.1M Bis-Tris-Propane, 0.5M Succinic acid, pH 7.0