5N6I
Crystal structure of mouse cGAS in complex with 39 bp DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99988 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.530, 122.940, 179.980 |
| Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
| Resolution | 47.000 - 3.600 |
| R-factor | 0.20646 |
| Rwork | 0.204 |
| R-free | 0.25631 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ley |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.443 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.000 |
| High resolution limit [Å] | 3.600 |
| Number of reflections | 41980 |
| <I/σ(I)> | 9.4 |
| Completeness [%] | 99.0 |
| Redundancy | 5.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 0.1M Tris pH 8, 0.2M ammonium citrate pH 7, 27,5% w/v PEG3350 |
