5N0H
Crystal structure of NDM-1 in complex with hydrolyzed meropenem - new refinement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-22 |
| Detector | RAYONIX MX300HE |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 106.000, 106.000, 92.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.190 - 1.900 |
| R-factor | 0.157 |
| Rwork | 0.155 |
| R-free | 0.20008 |
| RMSD bond length | 0.044 |
| RMSD bond angle | 1.797 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.190 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 40390 |
| <I/σ(I)> | 999 |
| Completeness [%] | 96.1 |
| Redundancy | 999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 1M trisodium cacodylate, 0.1M sodium cacodylate , pH 6.5 |
