5N09
Crystal structure of L107C/A313C covalently linked dengue 2 virus envelope glycoprotein dimer in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 A11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 58.902, 180.598, 205.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.991 - 3.900 |
| R-factor | 0.3064 |
| Rwork | 0.304 |
| R-free | 0.34680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4utb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.281 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.270 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.686 | 1.965 |
| Rpim | 0.354 | 1.044 |
| Number of reflections | 19970 | 23344 |
| <I/σ(I)> | 2.4 | 0.7 |
| Completeness [%] | 96.9 | 98 |
| Redundancy | 5.1 | 4.9 |
| CC(1/2) | 0.891 | 0.508 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100mM Tris pH 8.5 20.7% PEG 4K |
