5MZA

The DBLb domain of PF11_0521 PfEMP1 bound to human ICAM-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.998
Spacegroup name	P 21 21 21
Unit cell lengths	68.639, 109.830, 112.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.190 - 2.780
R-factor	0.2063
Rwork	0.205
R-free	0.23560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.970
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	78.700
High resolution limit [Å]	2.780
Number of reflections	22210
<I/σ(I)>	8.2
Completeness [%]	99.8
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	10% (w/v) PEG 20000, 20% (v/v) PEG 500 and 0.1M Tris-BICINE (pH 8.5)