5MYV
Crystal structure of SRPK2 in complex with compound 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 188.790, 248.990, 146.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.900 |
| R-factor | 0.19988 |
| Rwork | 0.199 |
| R-free | 0.21694 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb id 2X7G |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.182 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.870 | |
| High resolution limit [Å] | 2.900 | |
| Rmerge | 0.138 | 0.957 |
| Number of reflections | 75969 | 11061 |
| <I/σ(I)> | 9.5 | 1.6 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 5.8 | 6.1 |
| CC(1/2) | 0.993 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.25 | 277.15 | 1.0 M ammonium sulfate and 0.1 M acetate pH 5.25 |
