5MY8
Crystal structure of SRPK1 in complex with SPHINX31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 74.883, 74.883, 310.129 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.740 - 1.700 |
| R-factor | 0.16585 |
| Rwork | 0.164 |
| R-free | 0.20020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.648 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.740 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.078 | 0.799 |
| Number of reflections | 57614 | 8123 |
| <I/σ(I)> | 14.2 | 2.7 |
| Completeness [%] | 99.2 | 99 |
| Redundancy | 7.6 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.15 | 22% broad-molecular-weight PEG smears (BMW PEG smears) and 0.1 M citrate, pH 5.5 |
