5MXA

Structure of unbound Interleukin-23

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 61
Unit cell lengths	109.849, 109.849, 87.735
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.495 - 2.501
R-factor	0.1914
Rwork	0.188
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	IL-23 from pdb 4OE8
RMSD bond length	0.003
RMSD bond angle	0.730
Data reduction software	XDS (20161205)
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2614: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.690	2.650
High resolution limit [Å]	2.500	2.500
Rmeas	0.066	1.031
Number of reflections	20825	3297
<I/σ(I)>	24.59	2.24
Completeness [%]	99.7	98.2
Redundancy	10.27	10.14
CC(1/2)	1.000	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.3	287.15	100 mM citrate, 100 mM sodium phosphate, 20.5 % PEG 1000, 200 mM lithium sulphate