5MVN
X-ray structure of the M205W mutant of GLIC in complex with propofol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-05-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.229, 132.474, 159.759 |
| Unit cell angles | 90.00, 102.50, 90.00 |
Refinement procedure
| Resolution | 49.280 - 3.490 |
| R-factor | 0.2183 |
| Rwork | 0.218 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hfi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.490 |
| Number of reflections | 33576 |
| <I/σ(I)> | 3.5 |
| Completeness [%] | 98.0 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 400 mM NaSCN 100 mM NaAcetate pH4 12-15 % PEG4K 16 % glycerol 2 % DMSO |
