5MLU

Crystal structure of the PFV GAG CBS bound to a mononucleosome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97626
Spacegroup name	P 21 21 21
Unit cell lengths	107.260, 109.450, 175.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.607 - 2.800
R-factor	0.2094
Rwork	0.208
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kx5
RMSD bond length	0.003
RMSD bond angle	0.540
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (phenix)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.610	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.067	0.860
Number of reflections	51308
<I/σ(I)>	12.4	1.6
Completeness [%]	99.2	97.5
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.05M MnCl2, 0.04M KCl, 0.1M potassium cacodylate pH 6.0, 5% trehalose, 24% MPD