5MLE

Crystal Structure of Human Dihydropyrimidinease-like 2 (DPYSL2A)/Collapsin Response Mediator Protein (CRMP2 13-516) Mutant Y479E/Y499E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.920
Spacegroup name	C 2 2 21
Unit cell lengths	70.900, 185.820, 196.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.980 - 2.480
R-factor	0.20851
Rwork	0.206
R-free	0.25408
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gse
RMSD bond length	0.014
RMSD bond angle	1.667
Data reduction software	xia2
Data scaling software	xia2
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	83.980
High resolution limit [Å]	2.480
Number of reflections	46370
<I/σ(I)>	6.21
Completeness [%]	99.9
Redundancy	6.8
CC(1/2)	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	(0.2M magnesium chloride, 25% PEG 3350 and 0.1M bis-tris buffer pH 6.5