5MI8
Structure of the phosphomimetic mutant of EF-Tu T383E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-04 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.8696 |
Spacegroup name | P 1 |
Unit cell lengths | 55.870, 61.510, 65.040 |
Unit cell angles | 71.53, 71.17, 87.76 |
Refinement procedure
Resolution | 52.750 - 2.180 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.270 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 52.800 |
High resolution limit [Å] | 2.180 |
Rmeas | 0.105 |
Number of reflections | 38398 |
<I/σ(I)> | 8.3 |
Completeness [%] | 90.0 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M MgCl2, 0.1 M HEPES and 10 % PEG4000 |