5MI0
A thermally stabilised version of Plasmodium falciparum RH5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.656, 85.547, 132.873 |
| Unit cell angles | 90.00, 90.91, 90.00 |
Refinement procedure
| Resolution | 32.910 - 2.350 |
| R-factor | 0.1786 |
| Rwork | 0.176 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u0r |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.770 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.096 | 0.554 |
| Number of reflections | 36539 | |
| <I/σ(I)> | 13.9 | 3.1 |
| Completeness [%] | 98.6 | 97.9 |
| Redundancy | 6.5 | 6.9 |
| CC(1/2) | 0.996 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 18% glycerol 22% PEG 1500 and additive: 0.02M HEPES sodium pH 6.8 and benzidine nicotinamide, pyromellitic acid and sulfaguanidine, each at 0.25% w/v |
