5MF4

Tubulin-Dictyostatin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	104.010, 156.510, 179.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.854 - 2.300
R-factor	0.1859
Rwork	0.183
R-free	0.23250
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4i4t
RMSD bond length	0.008
RMSD bond angle	0.928
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.854	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.111	4.250
Number of reflections	129857
<I/σ(I)>	18.7	0.7
Completeness [%]	99.8	98.9
Redundancy	13.4	12.6
CC(1/2)	1.000	0.262

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	5% PEG, 12% GLYCEROL, 30 MM MAGNESIUM CHLORIDE, 30 MM CALCIUM CHLORIDE, 0.1M MES/0.1M IMIDAZOLE