5ME8

N-terminal domain of the human tumor suppressor ING5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	120
Detector technology	PIXEL
Collection date	2012-10-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978861
Spacegroup name	P 65 2 2
Unit cell lengths	135.630, 135.630, 96.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.700 - 3.200
R-factor	0.213
Rwork	0.211
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bkc 4afl
RMSD bond length	0.009
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	3.390
High resolution limit [Å]	3.200	3.200
Rmerge	0.084	0.850
Number of reflections	9086
<I/σ(I)>	47.54	6.2
Completeness [%]	99.9	99
Redundancy	7.6	35
CC(1/2)		0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	273	22 % MPD, 0.1 M MES pH 6.5, 5% PEG 3350