5M80

Translation initiation factor 4E in complex with (RP)-iPr-m7GppSpG mRNA 5' cap analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 1
Unit cell lengths	38.237, 38.271, 146.818
Unit cell angles	87.53, 95.37, 102.94

Refinement procedure

Resolution	37.290 - 2.120
R-factor	0.2066
Rwork	0.204
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l8b
RMSD bond length	0.008
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.120	2.120
Rmerge	0.068	0.417
Number of reflections	43091
<I/σ(I)>	10.47	2
Completeness [%]	93.9	89.5
Redundancy	1.979	1.829
CC(1/2)	0.996	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	19% PEG 10000, 0.2M CH3COONa, 0.1M TRIS pH 8.0