5M5E
Crystal structure of a interleukin-2 variant in complex with interleukin-2 receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 |
| Unit cell lengths | 127.965, 127.965, 135.073 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 111.070 - 2.300 |
| R-factor | 0.20053 |
| Rwork | 0.199 |
| R-free | 0.23487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2erj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.534 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 111.070 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 55462 |
| <I/σ(I)> | 8.7 |
| Completeness [%] | 99.7 |
| Redundancy | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES, pH 7.0, 2M ammonium sulfate |
