5M59

Crystal structure of Chaetomium thermophilum Brr2 helicase core in complex with Prp8 Jab1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 1 21 1
Unit cell lengths	92.540, 269.589, 231.689
Unit cell angles	90.00, 90.10, 90.00

Refinement procedure

Resolution	49.134 - 3.200
R-factor	0.2464
Rwork	0.244
R-free	0.28680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kit
RMSD bond length	0.002
RMSD bond angle	0.517
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.390
High resolution limit [Å]	3.200	3.200
Rmeas	0.295
Number of reflections	184926
<I/σ(I)>	8.1
Completeness [%]	98.8
Redundancy	7	6.8
CC(1/2)	0.991	0.266

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	277	0.2 mM spermine-tetrahydrochloride 3 % (v/v) Tacsimate, pH 7.0 2% (w/v) PEG MME5000 0.1 M HEPES-NaOH, pH 7.1