5LXF

Crystal structure of the human Macrophage Colony Stimulating Factor M- CSF_C31S variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	33.271, 65.471, 158.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.660 - 2.000
R-factor	0.1917
Rwork	0.189
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uf2
RMSD bond length	0.009
RMSD bond angle	1.176
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.660	2.030
High resolution limit [Å]	2.000	2.000
Rpim	0.025
Number of reflections	20774
<I/σ(I)>	26.5	1
Completeness [%]	95.1	85.7
Redundancy	8.7	8.2
CC(1/2)		0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	0.03M Bis-Tris pH 6.5; 0.17M Mg Formate; 16.67% PEG 3350; 0.07M Bis-Tris pH 5.5