5LV5

Crystal structure of mouse PRMT6 in complex with inhibitor LH1458

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9801
Spacegroup name	I 41
Unit cell lengths	77.703, 77.703, 117.692
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.160 - 1.802
R-factor	0.1561
Rwork	0.154
R-free	0.19520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c07
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11rc1_2513: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.160	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.105	1.261
Number of reflections	32113
<I/σ(I)>	10.7	1.2
Completeness [%]	99.8	97.3
Redundancy	5.2	4.7
CC(1/2)	0.998	0.421

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Tris-HCl pH 8.0 100 mM PEG 6000 10 % MgCl2 200 mM