5LV3

Crystal structure of mouse CARM1 in complex with ligand LH1561Br

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-11
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.8726
Spacegroup name	P 21 21 2
Unit cell lengths	75.092, 98.524, 208.097
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.992 - 1.800
R-factor	0.175
Rwork	0.173
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.005
RMSD bond angle	0.814
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_2386: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.820
High resolution limit [Å]	1.800	1.800
Rmerge	0.156	1.867
Number of reflections	143500
<I/σ(I)>	8.7	1.1
Completeness [%]	100.0	100
Redundancy	7.9	7.9
CC(1/2)	0.997	0.302

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	297	Tris-HCl pH 8.0 100 mM PEG 2000 MME 15% NaCl 100 mM