5LSJ

CRYSTAL STRUCTURE OF THE HUMAN KINETOCHORE MIS12-CENP-C delta-HEAD2 COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	68.160, 156.400, 76.000
Unit cell angles	90.00, 102.82, 90.00

Refinement procedure

Resolution	19.993 - 3.250
R-factor	0.2488
Rwork	0.246
R-free	0.29710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lsk
RMSD bond length	0.004
RMSD bond angle	0.815
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.500		3.080
High resolution limit [Å]	3.000	13.420	3.000
Rmerge	0.351	0.085	1.026
Number of reflections	31073
<I/σ(I)>	9.47	32.6	3.89
Completeness [%]	99.9	96	100
Redundancy	23.7		24.82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	20% PEG3350, 0.2M potassium sodium tartrate