5LP6
Crystal structure of Tubulin-Stathmin-TTL-Thiocolchicine Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2015-12-04 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.619, 155.285, 180.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.850 - 2.900 |
| R-factor | 0.27429 |
| Rwork | 0.273 |
| R-free | 0.30536 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o2b |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.103 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.850 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.128 | 1.428 |
| Number of reflections | 65141 | |
| <I/σ(I)> | 17.4 | 1.9 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 13.3 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Crystals grew overnight in precipitant solution consisting of 6.5% (v/v) polyethylene glycol 4000, 4% (v/v) glycerol, 0.1 M MES, 30 mM CaCl2 and 30 mM MgCl2 (pH 6.6) reaching their maximum dimensions within few days |
