5LP6

Crystal structure of Tubulin-Stathmin-TTL-Thiocolchicine Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Tubulin alpha-1B chain	polymer	440	48966.3	2	UniProt (P81947) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B, D (B, D)	Tubulin beta-2B chain	polymer	445	49999.9	2	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)
3	E (E)	Stathmin-4	polymer	143	16844.2	1	UniProt (P63043) Pfam (PF00836)	Rattus norvegicus (Rat)	Stathmin-like protein B3,RB3
4	F (F)	Uncharacterized protein	polymer	384	44378.5	1	UniProt (E1BQ43)	Gallus gallus (Chicken)
5	G, S (A, C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
6	H, J, K, L, N... (A, B, C)	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
7	I (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
8	M, V (B, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
9	O (B)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
10	P (B)	~{N}-[(7~{R})-1,2,3-trimethoxy-10-methylsulfanyl-9-oxidanylidene-6,7-dihydro-5~{H}-benzo[a]heptalen-7-yl]ethanamide	non-polymer		415.5	1	Chemie (71P)
11	U (C)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
12	AA, BA, W, X, Y... (E, F, A, B, C...)	water	water		18.0	89	Chemie (HOH)

Sequence modifications

E: 3 - 145 (UniProt: P63043)

PDB	External Database	Details
Met 3	Ile 74	conflict
Ala 4	Ser 75	conflict

F: 1 - 378 (UniProt: E1BQ43)

PDB	External Database	Details
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	259155.1
Non-Polymers*	Number of molecules	16
	Total formula weight	2813.5
All*	Total formula weight	261968.6

*Water molecules are not included.