5LLV
Crystal structure of DACM F87M/L110M Transthyretin mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2015-09-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.441, 83.760, 86.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.040 - 1.700 |
| R-factor | 0.15328 |
| Rwork | 0.150 |
| R-free | 0.22308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gko |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.679 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.040 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.111 | 0.772 |
| Number of reflections | 52003 | |
| <I/σ(I)> | 8.1 | 1.9 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.2 M CaCl2 0.1 M Sodium Acetate pH 4.6 20% v/v 2-propanol |
