5LLI

pVHL:EloB:EloC in complex with VH298

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9282
Spacegroup name	P 41 2 2
Unit cell lengths	94.643, 94.643, 368.582
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.550 - 2.400
R-factor	0.199
Rwork	0.196
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vcb
RMSD bond length	0.009
RMSD bond angle	1.327
Data reduction software	XDS
Data scaling software	Aimless (0.3.11)
Phasing software	REFMAC (5.8.0073)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.550	49.550	2.460
High resolution limit [Å]	2.400	11.260	2.400
Rmerge	0.099	0.026	0.850
Number of reflections	66540
<I/σ(I)>	12.5	37.1	2.3
Completeness [%]	99.6	98.9	99.5
Redundancy	6.6	5.9	7
CC(1/2)	0.999	1.000	0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	PEG 3350, MgOAc, Sodium cacodylate, DTT