5LHQ
The EGR-cmk active site inhibited catalytic domain of murine urokinase-type plasminogen activator in complex with the allosteric inhibitory nanobody Nb7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.04 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 94.250, 94.250, 122.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.811 - 2.600 |
R-factor | 0.1795 |
Rwork | 0.177 |
R-free | 0.21720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5LHR and 4JVP |
RMSD bond length | 0.013 |
RMSD bond angle | 1.517 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.820 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.087 | 0.966 |
Number of reflections | 19919 | |
<I/σ(I)> | 21.91 | |
Completeness [%] | 99.9 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 288 | 100 mM HEPES, 1.6 M Li2SO4 |