5LHC
The structure of D456A mutant of Nt.BspD6I nicking endonuclease at 0.24 nm resolution .
Replaces: 4WL5Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.96863 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.350, 92.666, 113.969 |
Unit cell angles | 90.00, 105.75, 90.00 |
Refinement procedure
Resolution | 19.000 - 2.400 |
R-factor | 0.19872 |
Rwork | 0.195 |
R-free | 0.26539 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ewf |
RMSD bond length | 0.015 |
RMSD bond angle | 1.780 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 109.700 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | 0.610 |
Number of reflections | 59826 | |
<I/σ(I)> | 9.8 | |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 3.6 | 3.5 |
CC(1/2) | 0.024 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 16% PEG 8000 40MM POTASSIUM PHOSPHATE 20% GLYCEROL |