5LFV
Crystal structure of glycosylated Myelin-associated glycoprotein (MAG) Ig1-3 with soaked trisaccharide ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99998 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.605, 60.115, 79.471 |
| Unit cell angles | 71.86, 86.51, 82.95 |
Refinement procedure
| Resolution | 56.786 - 2.300 |
| R-factor | 0.2253 |
| Rwork | 0.224 |
| R-free | 0.25410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1url 4frw 1cs6 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.583 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.790 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.157 | 0.981 |
| Number of reflections | 32993 | |
| <I/σ(I)> | 5.9 | 1.6 |
| Completeness [%] | 97.5 | 95.7 |
| Redundancy | 4.5 | 4.3 |
| CC(1/2) | 0.985 | 0.565 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.05 M tri-sodium citrate, 1.2 M ammonium sulfate, 3 % (w/v) isopropanol |
