5LFV

Crystal structure of glycosylated Myelin-associated glycoprotein (MAG) Ig1-3 with soaked trisaccharide ligand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Myelin-associated glycoprotein	polymer	317	35014.3	2	UniProt (P20917) Pfam (PF08205) Pfam (PF13927)	Mus musculus (House Mouse)	Siglec-4a
2	C, D (C, D)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G22573RC)
3	E (E)	N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		674.6	1	In PDB GlyTouCan (G47921NM)
4	F, P (A, B)	alpha-D-mannopyranose	non-polymer		180.2	2	Chemie (MAN)
5	G, H, I, Q, R (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)
6	J (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7	K, L, M, N, O... (A, B)	SULFATE ION	non-polymer		96.1	9	Chemie (SO4)
8	W, X (A, B)	water	water		18.0	34	Chemie (HOH)

Sequence modifications

A, B: 20 - 325 (UniProt: P20917)

PDB	External Database	Details
Gly 18	-	expression tag
Ser 19	-	expression tag
Ala 326	-	expression tag
Ala 327	-	expression tag
Ala 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag
His 333	-	expression tag
His 334	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70028.7
Branched	Number of molecules	3
	Total formula weight	2172.0
Non-Polymers*	Number of molecules	17
	Total formula weight	2423.0
All*	Total formula weight	74623.6

*Water molecules are not included.