5LF5

Myelin-associated glycoprotein (MAG) deglycosylated full extracellular domain with co-purified ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99998
Spacegroup name	P 32 2 1
Unit cell lengths	278.868, 278.868, 62.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.701 - 3.800
R-factor	0.2005
Rwork	0.199
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1url 4frw 1cs6 3p3y 2yd6
RMSD bond length	0.006
RMSD bond angle	1.397
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.700	4.030
High resolution limit [Å]	3.800	3.800
Rmerge	0.233	1.685
Number of reflections	27623
<I/σ(I)>	9.3	1.6
Completeness [%]	100.0	100
Redundancy	9.6	9.7
CC(1/2)	0.997	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	100 mM NaCl, 20 mM Tris/HCl pH 7.0, 7.7 % PEG 4000 (w/v)