5LA9

HIF prolyl hydroxylase 2 (PHD2-R281C/V314C) cross-linked to HIF-1alpha NODD-L397C/D412C and N-oxalylglycine (NOG) (complex-2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	88.467, 97.329, 71.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.129 - 2.813
R-factor	0.2525
Rwork	0.251
R-free	0.27850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hqr
RMSD bond length	0.003
RMSD bond angle	0.612
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.129	2.900
High resolution limit [Å]	2.810	2.810
Rmerge	0.245	1.250
Number of reflections	15152
<I/σ(I)>	4.7769	1.7378
Completeness [%]	98.5	99.3
Redundancy	3.4	3.5
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES pH 7.5, 2.0 M ammonium sulfate