5LA4
Crystal structure of apo human proheparanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.501, 88.061, 120.497 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.580 - 1.900 |
R-factor | 0.18743 |
Rwork | 0.185 |
R-free | 0.22729 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e8m |
RMSD bond length | 0.012 |
RMSD bond angle | 1.572 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.580 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.038 | 0.397 |
Number of reflections | 43065 | |
<I/σ(I)> | 28 | 5.3 |
Completeness [%] | 99.7 | 99.5 |
Redundancy | 7.9 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M Succinate pH 7.0 17% PEG3350 1:250 dilution seed stock - Seed stock grown in 0.2M Ammonium Nitrate, 20% PEG3350 |