5L9X

Human DNA polymerase eta-DNA ternary complex: reaction with 10 mM Mn2+ for 60s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.290, 98.290, 82.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.942 - 1.900
R-factor	0.1683
Rwork	0.165
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecv
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.081	0.016	0.684
Number of reflections	35503
<I/σ(I)>	20.9	90.3	3.08
Completeness [%]	99.9	91.6	99.8
Redundancy	6.9
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	17% PEG 2000MME, 0.1 M MES