5L8H
Structure of USP46-UbVME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 91.950, 104.660, 135.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.410 - 1.850 |
| R-factor | 0.16757 |
| Rwork | 0.166 |
| R-free | 0.19411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ibi |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.758 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.410 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.120 | 0.484 |
| Number of reflections | 55291 | |
| <I/σ(I)> | 17.68 | 2 |
| Completeness [%] | 98.8 | 99.7 |
| Redundancy | 3.56 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.96M Sodium Citrate pH7.5 and 0.1mM zinc chloride Cryo - 20% Glycerol |
