5L6E

Crystal structure of the human METTL3-METTL14 complex bound to SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.03965
Spacegroup name	P 32 2 1
Unit cell lengths	63.949, 63.949, 225.858
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.611 - 1.901
R-factor	0.1585
Rwork	0.157
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l6d
RMSD bond length	0.011
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.620	2.020
High resolution limit [Å]	1.900	1.900
Rmerge		0.706
Number of reflections	43352
<I/σ(I)>	29.93	4.71
Completeness [%]	99.9	99.5
Redundancy	19.5	19.2
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	22 % PEG 3350, 300 mM Mg acetate