5L6E

Crystal structure of the human METTL3-METTL14 complex bound to SAM

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001510	biological_process	RNA methylation
A	0001734	molecular_function	mRNA m(6)A methyltransferase activity
A	0005634	cellular_component	nucleus

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	binding site for residue SAM A 601

Chain	Residue
A	CYS376
A	LYS513
A	PHE534
A	ARG536
A	HIS538
A	ASN539
A	ASN549
A	GLN550
A	HOH712
A	HOH744
A	HOH778
A	ASP377
A	HOH805
A	ILE378
A	ASP395
A	PRO397
A	GLY407
A	LEU409
A	SER511
A	HIS512

---

site_id	AC2
Number of Residues	5
Details	binding site for residue MG A 602

Chain	Residue
A	ASP552
A	HOH708
A	HOH803
A	HOH829
A	HOH832

---

site_id	AC3
Number of Residues	6
Details	binding site for residue ACT B 401

Chain	Residue
A	GLU438
B	ARG249
B	ARG254
B	ARG255
B	HOH507
B	HOH602

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Region: {"description":"Interaction with METTL14","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Region: {"description":"Gate loop 2","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27373337","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27627798","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5L6D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5L6E","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27373337","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7W","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Site: {"description":"Interaction with METTL14","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	14
Details	Region: {"description":"Interaction with METTL3","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	9
Details	Region: {"description":"Positively charged region required for RNA-binding","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Site: {"description":"Interaction with METTL3","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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