5KXA
Selective Inhibition of Autotaxin is Effective in Mouse Models of Liver Fibrosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 133 |
| Detector technology | CCD |
| Collection date | 2015-03-11 |
| Detector | RAYONIX MX-325 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.100, 55.506, 70.075 |
| Unit cell angles | 71.56, 79.75, 81.09 |
Refinement procedure
| Resolution | 65.840 - 2.590 |
| R-factor | 0.17719 |
| Rwork | 0.173 |
| R-free | 0.24733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zga |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.678 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 65.840 |
| High resolution limit [Å] | 2.590 |
| Number of reflections | 23660 |
| <I/σ(I)> | 7.1 |
| Completeness [%] | 98.4 |
| Redundancy | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 20% PEG 3350, 0.1 M Sodium Acetate pH 7 |
