5KUE

Human SeMet incorporated I141M/L146M mitochondrial calcium uniporter (residues 72-189) crystal structure with magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-18
Detector	RAYONIX MX-300
Wavelength(s)	0.979
Spacegroup name	P 65
Unit cell lengths	55.500, 55.500, 69.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.443 - 1.500
R-factor	0.2017
Rwork	0.201
R-free	0.21240
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.398
Data reduction software	iMOSFLM
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.060
High resolution limit [Å]	1.500
Rmerge	0.094
Number of reflections	19352
<I/σ(I)>	15.1
Completeness [%]	100.0
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.32 M magnesium chloride, 0.1 M Tris pH 8.5, 24 % PEG 400, 1.6 M ammonium sulfate