5KT5

Teranry complex of human DNA polymerase iota R96G inserting dCMPNPP opposite template G in the presence of Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 65 2 2
Unit cell lengths	98.111, 98.111, 202.761
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.483 - 2.798
R-factor	0.2258
Rwork	0.224
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.629
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.483	50.000	2.850
High resolution limit [Å]	2.798	7.590	2.800
Rmerge	0.117	0.047	0.842
Number of reflections	14993
<I/σ(I)>	6
Completeness [%]	99.9	99.3	99.9
Redundancy	21	17.5	21.3
CC(1/2)		0.999	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	18% PEG MME 5000, 0.1M MES buffer, 0.3M ammonium sulfate