5KKL
Structure of ctPRC2 in complex with H3K27me3 and H3K27M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2016-06-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.254, 80.559, 133.220 |
| Unit cell angles | 90.00, 107.42, 90.00 |
Refinement procedure
| Resolution | 48.740 - 2.940 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.23370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kjh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Rmerge | 0.077 | 1.450 |
| Rpim | 0.032 | |
| Number of reflections | 36440 | 1739 |
| <I/σ(I)> | 24.15 | 1.36 |
| Completeness [%] | 98.0 | 94.7 |
| Redundancy | 6.5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG4000, 175mM ammonium citrate, pH 7.0 |
