5KJ8

Structure of the Ca2+-bound synaptotagmin-1 SNARE complex (long unit cell form) - from synchrotron diffraction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	68.790, 169.710, 286.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.640 - 4.100
R-factor	0.284
Rwork	0.283
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n7s 3f04 1uow
RMSD bond length	0.002
RMSD bond angle	0.607
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.210
High resolution limit [Å]	4.100	4.100
Rmerge	0.120	0.780
Number of reflections	27282
<I/σ(I)>	7.6	1.8
Completeness [%]	98.1	99.1
Redundancy	3.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	0.25% v/v PEG8000, 25 mM HEPES-Na, 75 mM NaCl, 25 mM MgCl2, 0.25 mM CaCl2