Structure of the Ca2+-bound synaptotagmin-1 SNARE complex (long unit cell form) - from synchrotron diffraction

Summary for 5KJ8

DescriptorVesicle-associated membrane protein 3, Syntaxin-1A, Synaptosomal-associated protein 25, ... (6 entities in total)
Functional Keywordsxfel structure, synaptic fusion complex, synaptotagmin1, neuronal snare complex, endocytosis, exocytosis
Biological sourceRattus norvegicus (Rat)
Cellular locationMembrane ; Single-pass type IV membrane protein  P63025
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass type IV membrane protein  P32851
Cytoplasm, perinuclear region  P60881 P60881
Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein  P21707
Total number of polymer chains11
Total molecular weight158567.05
Lyubimov, A.Y.,Uervirojnangkoorn, M.,Zhou, Q.,Zhao, M.,Sauter, N.K.,Brewster, A.S.,Weis, W.I.,Brunger, A.T. (deposition date: 2016-06-17, release date: 2016-10-19, Last modification date: 2016-10-26)
Primary citation
Lyubimov, A.Y.,Uervirojnangkoorn, M.,Zeldin, O.B.,Zhou, Q.,Zhao, M.,Brewster, A.S.,Michels-Clark, T.,Holton, J.M.,Sauter, N.K.,Weis, W.I.,Brunger, A.T.
Advances in X-ray free electron laser (XFEL) diffraction data processing applied to the crystal structure of the synaptotagmin-1 / SNARE complex.
Elife, 5:-, 2016
PubMed: 27731796 (PDB entries with the same primary citation)
DOI: 10.7554/eLife.18740
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.3017000.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report