5KI6
Human Argonaute-2 bound to a guide RNA with a nucleobase modification at position 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2014-09-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.276, 107.297, 68.855 |
| Unit cell angles | 90.00, 107.44, 90.00 |
Refinement procedure
| Resolution | 47.574 - 2.153 |
| R-factor | 0.2391 |
| Rwork | 0.237 |
| R-free | 0.26970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ola |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.045 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.570 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.036 | 0.492 |
| Number of reflections | 47203 | |
| <I/σ(I)> | 18.1 | 2.2 |
| Completeness [%] | 99.3 | 97.2 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | PEG 3350, Phenol, Tris, Spermine, Isopropanol |
