5KE8

mouse Klf4 E446P ZnF1-3 and MpG/MpG sequence DNA complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-04
Detector	RAYONIX MX300-HS
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	50.450, 50.450, 130.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.674 - 2.450
R-factor	0.233
Rwork	0.229
R-free	0.26970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m9e
RMSD bond length	0.005
RMSD bond angle	0.698
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2400: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.674	2.510
High resolution limit [Å]	2.450	2.450
Rmerge	0.060	0.201
Number of reflections	4805
<I/σ(I)>	17.88	6.31
Completeness [%]	71.7	4.8
Redundancy	3.8	4.5
CC(1/2)	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	0.1M Tris-HCl pH8.5 , 0.25M NaCl and 20% polyethylene glycol 8000