5KA2
Protein Tyrosine Phosphatase 1B YAYA (Y152A, Y153A) mutant, open state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-02 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 87.306, 87.306, 98.625 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.918 - 2.071 |
| R-factor | 0.2053 |
| Rwork | 0.204 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.511 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.110 |
| High resolution limit [Å] | 2.070 | 5.620 | 2.070 |
| Rmerge | 0.106 | 0.051 | 0.725 |
| Total number of observations | 286716 | ||
| Number of reflections | 26905 | ||
| <I/σ(I)> | 6.6 | ||
| Completeness [%] | 99.8 | 99.6 | 98.8 |
| Redundancy | 10.7 | 10.1 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Tris, pH 8.0, 0.2 M MgCl2, 24% PEG8000 |
