5K8X
Crystal structure of mouse CARM1 in complex with inhibitor U3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.831, 98.486, 205.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.555 - 1.991 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ih3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.002 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1980) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.240 | 2.010 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.161 | |
| Number of reflections | 103982 | |
| <I/σ(I)> | 7.3 | 0.8 |
| Completeness [%] | 99.2 | 84.8 |
| Redundancy | 6.6 | 5.7 |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Tris-HCl pH 8.0 100 mM, PEG 2000 MME 15 %, NaCl 100 mM |
