5K78

Dbr1 in complex with 16-mer branched RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.28081
Spacegroup name	P 21 21 21
Unit cell lengths	73.893, 142.673, 215.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.336 - 2.640
R-factor	0.1972
Rwork	0.195
R-free	0.24560
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4pef
RMSD bond length	0.002
RMSD bond angle	0.545
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	108.000	2.780
High resolution limit [Å]	2.640	2.640
Rmerge	0.151	1.760
Number of reflections	66902
<I/σ(I)>	10.3	1.1
Completeness [%]	99.1	99.8
Redundancy	5.6	5.8
CC(1/2)	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	PEG 3350, lithium sulfate, glycerol, bis-tris