5K5L
Homo sapiens CCCTC-binding factor (CTCF) ZnF6-8 and H19 sequence DNA complex structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-26 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.2829 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 126.792, 52.397, 69.135 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.725 - 3.125 |
R-factor | 0.2379 |
Rwork | 0.236 |
R-free | 0.27020 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.535 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((dev_2313: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.725 | 3.210 |
High resolution limit [Å] | 3.125 | 3.125 |
Rmerge | 0.114 | 0.392 |
Number of reflections | 15139 | |
<I/σ(I)> | 14.917 | 3.556 |
Completeness [%] | 98.7 | 91.1 |
Redundancy | 7.5 | 4.8 |
CC(1/2) | 0.980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 289 | 20% (w/v) polyethylene glycol 8,000 0.1 M CHES-NaOH pH 9.5 |